Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery

Therapeutic Angiogenesis by Ultrasound-Mediated MicroRNA-126-3p Delivery.

OBJECTIVE MicroRNAs are involved in many critical functions, including angiogenesis. Ultrasound-targeted microbubble destruction (UTMD) is a noninvasive technique for targeted vascular transfection of plasmid DNA and may be well suited for proangiogenic microRNA delivery. We aimed to investigate UTMD of miR-126-3p for therapeutic angiogenesis in chronic ischemia. APPROACH AND RESULTS The angi...

Antagomir-mediated silencing of endothelial cell specific microRNA-126 impairs ischemia-induced angiogenesis

MicroRNAs are negative regulators of gene expression that play a key role in cell-type specific differentiation and modulation of cell function and have been proposed to be involved in neovascularization. Previously, using an extensive cloning and sequencing approach, we identified miR-126 to be specifically and highly expressed in human endothelial cells (EC). Here, we demonstrate EC-specific ...

MicroRNA-126-3p suppresses cell proliferation by targeting PIK3R2 in Kaposi's sarcoma cells

Kaposi's sarcoma is a highly vascular tumor of lymphatic endothelial origin. Many deregulated miRNAs, including miR-126-3p, have been identified in Kaposi's sarcoma tissues. miR-126-3p is the most highly endothelial-specific miRNA that regulates vascular integrity and angiogenesis. In this study, we aimed to determine the effect of miR-126-3p on Kaposi's sarcoma cells through transfection of a ...

Ultrasound-mediated Drug Delivery

Ultrasound-Mediated Gene Delivery

Human gene therapy holds great promise in treating not only hereditary genetic disorders, but also disease states such as cancer and viral infections, and contingencies such as stroke or myocardial infarctions. It can be achieved by delivery of a correct gene into target cells with genetic deficiency or mutations, or by transfer of a therapeutic agent such as agents targeting a cancer-causing o...